Dr. ISIBOR CLEMENT NDUDI
Publication Title Bacterial Associated with Swimming Pool Water in Benin City Nigeria
Publication Type journal
Publisher African Journal of Health, Safety and Environment
Publication Authors Okoruwa, A. I. , Isibor, C.N and Ukpene, A. O (2023
Year Published 3-03
Abstract
Publication Title Comparative In-Vitro Activity of Fluoroquinolones Against Clinical Bacterial Pathogens Isolated from Central Hospital, Warri
Publication Type journal
Publisher Nigerian Journal of Scientific Research, 21(1): 1 -10.
Publication Authors ISIBOR, C. N. and Ahonkhai I., (2022).
Year Published 4-11
Abstract Local sensitivity and resistance are important guides to help clinicians to make good choices in a given situation
that demands the use of antimicrobial agents. Antibiotic susceptibility testing was carried on bacterial isolates
from clinical specimens and ascertain their susceptibility to fluoroquinolones and other antimicrobial agents. A
total of 260 clinical samples of urine, Sputum, cerebrospinal fluid, semen, aspirates, and swabs from wound,
virginal, ear, and nose consecutively at the Central Hospital, Warri was examined using standard bacteriological
techniques. Antibiotic susceptibility tests were performed using the standard disc diffusion method. A total of 118
bacterial isolates were recovered. The significant pathogens included Staphylococcus aureus (31.35%), Klebsiella
aerogenes (27.97%), Escherichia coli (22.03%), Pseudomonas aeruginosa (9.30%), Proteus mirabilis. (8.5%)
and Streptococci sp. (0.85%). Overall sensitivity was shown to be pefloxacin 100% while ofloxacin, ceftazidime
and cefuroxime were 83.05% and ciprofloxacin 80.51%, gentamicin 77.12%, streptomycin 34.75%, penicillin
7.6% and ampicillin 2.5% of strains tested. The sensitivity results showed the continued increase in the prevalence
of antibiotic resistance in older penicillin-based B-lactams, penicillin and ampicillin. Also, given the prominent
role of susceptibility in health matters, there is a need to keep physicians current on organisms' sensitivity and
resistance possibilities, as demonstrated by this work.
Publication Title Haematological Evaluation of Mycobacterium tuberculosis Subjects at Central Hospital Agbor, Nigeria
Publication Type journal
Publisher
Publication Authors ISIBOR, C. N. and ADU, M.E (2022)
Year Published 2022-06-01
Abstract Tuberculosis (TB) is a severe public health problem in Nigeria that is caused by Mycobacterium tuberculosis and has its attendant effects. The aim of this study was to evaluate the haematological profile of 146 newly diagnosed patients with Mycobacterium tuberculosis attending Central Hospital Agbor, Delta State. A total of 38 Mycobacterium tuberculosis negative individuals were monitored as control. Five millilitres of venous blood were collected into EDTA containers. Complete blood count (Packed cell volume (PCV), haemoglobin, total white cell count, lymphocyte, platelet and neutrophil counts) was estimated using Sysmex XP 300 – haematology analyser. SPSS was used to analyse the data. There was no significant difference (p>0.05) observed in the total white blood cell counts between tuberculosis subjects and control subjects. Tuberculosis patients had significantly lower (p <0>0.05) in the mean cell haemoglobin of tuberculosis and control subjects when compared. However, there was a significantly lower (p <0.05) mean cell haemoglobin concentration among the in tuberculosis-associated complications. tuberculosis patients compared to controls There was significantly higher (p <0.05) Neutrophil Lymphocyte Ratio (NLR) and Platelet-Lymphocyte Ratio (PLR) among the tuberculosis subjects when compared with controls. There were observed haematological abnormalities in tuberculosis subjects in the study area. The study identified that NLR and PLCR as novel biomarkers for monitoring systemic inflammation in tuberculosis. It is therefore pertinent to estimate haematological parameters early in tuberculosis subjects to help in informed clinical decision making in the management of these subjects.
Publication Title Pro-inflammatory cytokine profile of pulmonary tuberculosis patients in Central Hospital, Agbor Nigeria
Publication Type journal
Publisher
Paper Link https://doi.org/10.4103/ljms.ljms_41_19
Publication Authors ISIBOR C.N, OKOJIE, R.O, OPHORI, E.A., and OMONIGHO, S.E. (2020).
Year Published 2020-12-20
Abstract Background: The aim is to determine the pattern of cytokines secretion by assessing interleukins (IL?1, IL?6, and tumor necrosis
factor?alpha (TNF??) in pulmonary tuberculosis patients. Materials and Methods: A cross?sectional study was conducted on 146
consecutive (54 males and 92 females) sputum positive for tuberculosis and 38 apparently healthy age? and sex?matched sputum negative
for tuberculosis as control were recruited between May 2016 and June 2017. A volume of 5 mL of blood samples was collected for the
determination of serum IL?1, IL?6 and TNF?? using the ELISA method. Results: There was a higher cytokine mean ± standard error of
the mean for tuberculosis subjects (95.77 ± 6.68 pg/mL; 107.54 ± 14.76 pg/mL, 122.09 ± 16.55 pg/ml) and controls (79.88 ± 3.53 pg/ml;
78.35 ± 6.82 pg/ml; 94.11 ± 14.08 pg/ml) for interleukin?1, interleukin?6, and TNF??, respectively, when compared. There was strong correlation
between mean values of IL?6 and TNF?? (r = 0.72315, P < 0.05). There was significance difference (P < 0.05) observed in the mean serum
concentrations of cytokines among the genders (P < 0.05). Conclusion: The study revealed that IL?1, IL?6, TNF?? are important biological
markers for tuberculosis disease.
Publication Title Plasma Fibrinogen: A Cardiovascular Risk Biomarker in Type 2 Diabetes Mellitus Subjects.
Publication Type journal
Publisher
Paper Link https://doi.org/10.33003/fjs-2022-0603-915
Publication Authors ADU, M.E. and ISIBOR, C. N. (2022):
Year Published 2022-06-24
Abstract Diabetes mellitus is a public health disease considered as metabolic and endocrine disorder that has been
involved in cardiovascular episode. Fibrinogen, a glycoprotein has been incriminated as a risk factor of
cardiovascular disease. This study aims to evaluate the plasma fibrinogen concentration of type 2 diabetes
mellitus subjects in Agbor, Nigeria. A total of three hundred and ninety six (396) respondents were recruited
into the study which comprises of three hundred and six (306) type 2 diabetes mellitus and ninety (90) age and
sex matched apparently healthy subjects as controls. Five milliliters (5ml) of venous blood was collected
aseptically with minimum stasis and dispensed into appropriate containers. Plasma fibrinogen concentration
was evaluated using standard methods while statistical package for social sciences was employed to determine
difference in mean ± standard deviation using student t test. Diabetes mellitus subjects exhibit a significantly
(p<0>0.05) difference observed in the age
of all respondents. It is therefore pertinent to state that type 2 diabetes mellitus are prone to cardiovascular
disease as well as the male gender. It is therefore recommended that plasma fibrinogen should be done regularly
for diabetes mellitus subjects as well as life-style modification to prevent excess mortality.
Publication Title Seasonal Responses of Flowering and Pod Setting in Cowpea (Vigna unguiculata L. Walp)
Publication Type journal
Publisher
Paper Link https://doi.org/10.33003/fjs-2022-0603-915
Publication Authors Ukpene, A. O. and Isibor, C. N. (2022).
Year Published 2022-06-30
Abstract Seven varieties of cowpea, Vigna unguiculata L. Walp were studied in two field trials (the wet and dry seasons)
in the 2021 cropping season in Agbor, South-South geopolitical zone of Nigeria, were laid out in a complete
randomized block design (CRBD) with seven replications for seasonal responses of flowering and pod setting.
The dry season crops were manually watered twice daily while the wet season crops were rain-fed. The study
recorded that most of the cowpea genotypes grown in the wet season showed photoperiod sensitivity, flowering
between 46.36 and 56.43 DAP with mean of 54.97 + 6.98. Pod setting was similar across genotypes but the
number (9.64 + 8.29), while length of pods varied (14.66 + 2.83) as noted from field observations. Furthermore,
Seeds per pod was not significant among varieties of the wet season but they were significant and among those
of the dry season (9.37 + 5.17). Genotypes which flowered earlier produced higher number of pods per plant
and gave better yield such as IT06K-149-1, IT98K-128-3, IT95K1072-57 and IT98K-506-1 with grain filling
of 205.58, 186.3, 183.53 and 775.99 mean seeds respectively were
Publication Title Prevalence of Mycobacterium Tuberculosis in Human Immunodeficiency Virus Patients at ART Centre Agbor, Nigeria.
Publication Type journal
Publisher
Paper Link https://doi.org/10.5897/AJCPATH15.011
Publication Authors ADU M.E., EZIE, E. ISIBOR, C.N. and JEMIKALAJAH, D.J. (2015).
Year Published 2015-05-31
Abstract Aim: The prevalence of pulmonary tuberculosis (PTB) in Human immunodeficiency virus (HIV)/Acquired immunodeficiency syndrome (AIDS) patients is a dual epidemic problem of major concern worldwide especially in Africa where there is upsurge of the disease. This study is set to determine the prevalence of PTB among HIV patients.
Methods: A total of 489 patients were recruited into the study, 173(35.4%) were males, while 316 (64.6%) were females between January 2012 and December 2013. HIV status and Mycobacterium tuberculosis were determined using WHO systems two and Ziehl Neelsen (ZN) staining technique respectively.
Results: Prevalence of 10.6%, 79.6% and 9.8% was obtained for HIV/PTB co infection, HIV and PTB respectively. A prevalence of 5.1% recorded for HIV/PTB co infection is highest among age group 31-45years, followed by 2.7% and 1.6% in the age groups 16-30 and 46-60years respectively. The lowest prevalence of 0.2% each was observed in the age groups 61-75 and 76-90 years.
Conclusion: There is need for more advocacy and proper monitoring of infected patients to check the spread of HIV infections especially in a depressed economy like Nigeria in order to further reduce cases of PTB.
Publication Title Prevalence of Haemoglobin Variants Among the Ika Ethnic Nationality of Delta State
Publication Type journal
Publisher
Publication Authors ADU E.M, ISIBOR, C.N. and EZIE E. (2014).
Year Published 2014-07-31
Abstract Background: Haemoglobin genotype is an important blood
component that determines haemoglobinopathies. Distribution
of haemoglobin variants was investigated among the Ika ethnic
nationality of Delta State, Nigeria. Aim: The present study was
conducted to determine the prevalence of haemoglobin variants
and also to provide information for instituting genetic counseling
services to reduce haemoglobinopathies between couples.
Methods: A total of 600 subjects were screened for
haemoglobin genotypes using paper electrophoresis. Results:
The prevalence of HbAA, HbAS, HbAC and HbSS were 78%,
19.5%, 0.5% and 2% respectively. There was no relationship
(P?0.05) between sex and haemoglobin variants. There was a
low prevalence of abnormal haemoglobin variants observed
which is consistent with previous studies. Conclusion: The
sickle cell trait (HbAS) is the largest variant in this population;
we therefore advocate mandatory haemglobin genotype
screening for all intending couples in order to reduce the
sickling gene pool.
Publication Title Seroprevalence of Hepatitis B Virus among Human Immunodeficiency Virus Patients Attending Antiretroviral Treatment (ART) Centre, Agbor, Nigeria
Publication Type journal
Publisher
Publication Authors ADU E.M, ISIBOR C.N., EZIE. E. and BINI, E.N. (2012).
Year Published 2012-01-12
Abstract The prevalence of some infectious diseases (Hepatitis B) has changed with the incidence of HIV/AIDS pandemic. This study was carried out to determine the prevalence of hepatitis B virus (HBV) infection in human immunodeficiency virus (HIV) infected patients undergoing treatment in the Antiretroviral Treatment Centre Agbor, Delta State,Nigeria. A total of 538 HIV infected subjects were recruited for this study, comprising 156(29%) males and 382(71%) females aged between 1-70 years and were screened for Hepatitis B. Of the 538 samples , 12(7.7%) males and 8(2.0%) females were sero-positive for HBV. The highest seroprevalence (5.9%) was observed in the group aged 46-60 years, followed by the age group of 1-15 years (4.0%) and 31-45 years (3.5%). The lowest prevalence of (0%) was observed in the group aged 61-70 years. The difference in seroprevalence between the groups aged 1-15 years and 46-60 was statistically significant (P < 0.001) .Enlightenment campaigns should be intensified as well as immunisation against Hepatitis B in order to achieve Hepatitis B free population.
Publication Title Lymphocyte subpopulations among pregnant women in Agbor, Delta State Nigeria
Publication Type journal
Publisher
Publication Authors OPHORI, E.A and ISIBOR, N.C. (2012)
Year Published 2012-07-01
Abstract Objective
To determine the peripheral blood CD3 and CD8 T-lymphocytes subpopulation in healthy pregnant women in our environment.
Methods
Pregnant women attending the antenatal care clinic (ANC) of the Central Hospital, Agbor, Nigeria, who gave informed consent were recruited for the study. Blood samples (10 mL) was collected by venipuncture from 121 healthy pregnant women (aged 17-47 years; mean 28.5±0.43). Tweenty four non-pregnant healthy women (aged 18-30years; mean 21.12±0.64) were enrolled as control. CD3+ and CD8+ counts were determined with the Partec flow cytometer.
Results
The mean CD3+ count for pregnant women was 1311.10±71.50 ?/L when compared to the controls which was 9.67±0.99 ?/L. This was statistically significant(P<0>0.005). Primigravidas had a higher CD3+ mean count than multiparas while mean CD8+ count was higher in multiparas than in primigravidas. Similarly, the mean CD3+ count was significantly different between all the trimesters with the highest count of 1, 353.23±43.90 ?/L in the first trimester as compared to the 9.67±0.99 ?/L in the control. Mean CD3+ was highly significant in pregnant women with history of previous abortions while the CD8+ cells were not.
Conclusion
The results suggest the need for routine determination of T lymphocyte subsets apart from CD4 in pregnant women attending ANC clinic to prevent any possible immunological /pregnancy disorder during the period of pregnancy.
Publication Title Immunological response to Helicobacter pylori among healthy volunteers in Agbor, Nigeria
Publication Type journal
Publisher
Publication Authors 12. OPHORI, E.A., ISIBOR, C., ONEMU, S.O. and JOHNNY, E.J. (2011)
Year Published 2011-11-17
Abstract Objective
To determine Helicobacter pylori (H. pylori) immunologically.
Method
The seroprevalence of H. pylori infection was determined by ELISA technique in 380 individuals who gave informed consent, comprising 180 males (47.4%) and 200 females (52.6%) in Agbor, Nigeria. 124 (27.4%) of the subjects lived in the rural communities while 276 (72.6%) lived in the urban areas of Agbor. They were aged 1 to over 70 yr [(mean age±SD)=(39±9. 42) yr]. Five milliliter of clotted blood was obtained from each subject for specific HP-IgG antibodies.
Results
Antibodies to H. pylori were detected in serum of 159 males (88.3%) and 182 females (91.0%). This was not statistically significant (P> 0.05). A total of 341 (89.7±21.4%) subjects presented anti-H. pylori antibodies. The prevalence of H. pylori increased with age from 14.8% in age group 1–9 yr to 100% in age group 30-39 yr and 60–69 yr. At above 70 yr, the seroprevalence decreased to 93.3%.
Conclusions
The prevalence of H. pylori in Agbor is very high among adults while comparatively low among children. Since a lot of factors have been attributed to affect the rate of infection, increased standard of living and improved public health measures are encouraged and studies on the development and administration of an effective vaccine is recommended.
Publication Title Identification and antibiotics sensitivity test of bacteria from stool of patients with acute diarrhoea
Publication Type journal
Publisher
Paper Link https://doi.org/10.4314/jast.v15i1-2.54837
Publication Authors OPHORI E.A., ISIBOR, C., ISIBOR J.A and ADU M. (2010)
Year Published 2010-10-18
Abstract One hundred and fifty stool samples from 65 female and 85 male patients with acute diarrhoea from the Central Hospital, Agbor (Nigeria) were examined to ascertain the likelihood of cholera outbreak in Agbor. The samples were preserved in Carey-Blair semi-solid medium, inoculated directly on blood agar, McConkey agar, deoxycholate citrate (DCA) agar and thiosulphate-citrate-bile-salt (TCBS) agar, and incubated aerobically for 24 h at 37 º C. The isolates were characterised by sugar fermentation tests and cultural morphology. Seven bacterial genera were identified and Escherichia coli had an incidence of 58 %, Vibrio cholerae 36 %, Salmonella spp. 14.7 %, Aeromonas hydrophila 9.3 %, Shigella dysenteriae 7.3 % and Klebsiella oxytoca 7.3. % each and 3.3 % for Shigella flexneri. The V. cholerae isolates were designated VC01-VC07, and were serotyped using polyvalent, monospecific Ogawa and Inaba 01 and 0139 antisera. V. cholerae 01 biotype ETor serotype Ogawa was predo-minant and prevalent between April - May 2008, when most of the isolates were isolated. For the VC01, 39 iso-lates were from males (72.2 %) and 15 were from females (27.8 %). The bacteria isolates were tested against 9 antibiotics by the disc diffusion method. E. coli, Salmonella spp, Shigella spp and Aeromonas hydrophila were all resistant to gentamycin, tetracycline and penicillin (100 %) but were sensitive to chloramphenicol, ciprofloxacin and cefotaxime. All the V. cholerae strains (100 %) were highly sensitive to cephataxime, ciprofloxacin, chloramphenicol and erythromycin, but were resistant to streptomycin (71.4 %), tetracycline (71.4 %) and peni-cillin (57.1 %), while all isolates showed intermediate resistance or sensitivity to gentamycin. The study indicated that there was an ecological niche that supported V. cholerae 01 biotype in Agbor, which could become public health concern, unless detailed epidemiological investigation is conducted.
Publication Title Evaluation of the sodium hypochlorite concentration method of detecting acid-fast bacilli from sputum of patients in Central Hospital, Delta state, Nigeria
Publication Type journal
Publisher
Publication Authors ISIBOR C, OPHORI, E.A. and ISIBOR, J. (2009).
Year Published 2009-06-05
Abstract The use of sodium hypochlorite (NaOCl) was revalidated in the laboratory diagnosis of acid fast bacilli (AFB) against the long existing direct smear Zeihl-Neelsen method to ascertain the more sensitive and reliable method. Sputum from 260 patients, aged 20-52 years attending the Chest Clinic of the Central Hospital Agbor, Delta State, Nigeria were examined for acid-fast bacilli by direct microscopy and centrifugation following treatment with 3.5% sodium hypochlorite. The percentage positive cases rose from 6.92% for direct smear to 28.85% after treatment with sodium hypochlorite. This was found to be statistically significant (p<0.0001). The sodium hypochlorite concentration improved specificity from 43.33% to 56.67% and sensitivity from 19.35% to 80.65%. Thus, the sodium hypochlorite concentration method may be more sensitive and reliable for the diagnosis of tuberculosis in laboratories. Hence, it could be recommended for use in Tuberculosis Control Programme in Nigeria and can be adapted to suit local needs of rapid diagnosis.
Publication Title Evaluation of cd4+ and cd8+ lymphocyte counts in patients with tuberculosis infection
Publication Type journal
Publisher
Publication Authors ISIBOR, C. N, OMOINGHO, S. E. OPHORI, E.A, OKOJIE, R AND ADU. M.E (2021).
Year Published 2021-07-01
Abstract Background: Tuberculosis is a public health disease and has been observed to affect the immune system of individuals.
Aim: The effect of tuberculosis on the immune system has prompted this study to evaluate the CD4+ and CD8+ cells of individuals diagnosed with tuberculosis attending the Chest Clinic at Central Hospital, Agbor.
Method: A total of five hundred (500) suspected tuberculosis (Tb) subjects where screened and one hundred and forty six (146) confirmed Tb cases were used for the study. This comprises of 113 Tb cases only and 33 Tb/HIV co-infected cases while thirty-eight (38) healthy subjects was used as control with an age range of 8-75years and mean age of 35.41±1.15years were recruited. The sputa were examined using Genexpert to confirm Mycobacterium tuberculosis. Five millilitres (5ml) of blood were collected from subjects and analysed for CD4+, CD8+ cells using Partec CyFlow Counter. HIV Status was determined according to the Nigeria national HIV testing algorithm. Mean ±SEM were determined using statistical package for social sciences.
Results: A prevalence of 29.5% (146) was observed for tuberculosis with females 63% having the highest burden as against males with 37% while the age group 16-45years had the highest prevalence rate. The mean ± standard error of the mean of CD4+ was significantly (p<0>0.05) difference observed between co-infected and HIV subjects when compared.
Conclusion: It is therefore pertinent to state that CD4+ cannot be used as a biomarker for tuberculosis disease progression.
Publication Title CD4:CD8 T-cell Ratio and Full Blood Count Status of Human immunodeficiency Virus (HIV) and Pulmonary Tuberculosis (TB) Patients in Three Centers with Voluntary Testing and Counselling Units in Delta State, Nigeria
Publication Type journal
Publisher
Publication Authors D.J. JEMIKALAJAH, G.R.A. OKOGUN AND C.N. ISIBOR (2008).
Year Published 2028-10-09
Abstract Two hundred and five patients suspected of Human immunodeficiency virus (HIV) infection and or Pulmonary Tuberculosis (TB) and 100 apparently healthy subjects were recruited into the study from February 2006 to February 2008 to determine the relevance of CD4:CD8 T-cell ratio and full blood counts as diagnostic indices of the immunological status of HIV and TB subjects in parts of Delta State, Nigeria. HIV status was determined
using (WHO) System two and Ziehl Neelsen staining techniques were used for tuberculosis screening. Automatic Cyflow counter was employed for the estimation of CD4:CD8 ratio and standard haematological technique was used for full blood count measurements. The mean CD4 of 220.7 ?19.5, 445.1 ?33.0 and 205.2? 30.5 for HIV, TB and HIV/TB respectively were significantly different (p <0.05) when compared with 880.4 ? 49.3 for controls. Likewise, the mean CD8+ of 724.8 ? 106.0 for HIV and mean T-cell of 0.4 ?0.0 for HIV/TB infected subjects were significantly different P < 0.05) when compared with 401.6 22.3 and 2.8 for controls respectively. The mean PCV of 25.9 ?0.6, 27.5 0.5 and 25.0 0.9, mean neutrophil of 62.9 1.6, 62.6 1.4, 63.7 2.8; mean lymphocyte of 36.0 ?1.5, 34.7? 1.3 and 34.3? 7.0 for HIV, TB and HIV/TB were also significantly different (P < 0.05), when compared with 35.9? 0.3, 54.9 ? 0.9 and 14.5 0.9 or controls respectively. The mean eosinophil count of 6.7 ? 1.7 for tuberculosis was significantly higher (P < 0.05) when compared with 2.02 02 for controls this study further confirms CD4:CD8 + ratio and lymphocytes count as good diagnostic indices for HIV and TB infections in Delta State Nigeria.
Publication Title Adherence Capabilities of Bacterial Isolates from Human Conjunctivitis
Publication Type journal
Publisher
Publication Authors OPHORI, E.A., ADU, M, ISIBOR C. (2005).
Year Published 2005-02-01
Abstract The adherence capabilities of Staphylococcus aureus, Streptococcus pneumoniae, Pseudomonas aeruginosa and Haemophilus influenzae to liquid hydrocarbons and polystyrenes were studied as a means of assessing the cell surface hydrophobicity of the bacteria to the human conjunctiva. The bacteria were suspended in phosphate urea magnesium (PUM) and various volumes of the hydrocarbons were added to the cells suspension and the absorbance of the lower aqueous phase was read at 400m o determine the adherence to liquid hydrocarbons. Adherence to polystyrene was by the replica method of screening for bacteria hydrophobicity. Results showed that the bacteria adherence to hydrocarbons and the level of hydrophobicity varied with organism. The percentage mean adherence for Staphylococcus aureus ranged from 2.5-3.5%, that of P. aeruginosa was between 4-15%, Streptococcus pneumoniae 2.0-8.4% and Haemophilus influenzae was 1.7-12.6% to the liquid hydrocarbons used Ther was no correlation between the two methods. S. aureus and P. aeruginosa adhered well to polystyrene while H. influenzae and S. pnuemoniae adherence was poor. Adherence was generally poor in all cases, therefore hydrophobicity may not possibly play a significant role in adherence to the conjunctiva
Publication Title Prevalence of Malaria Parasitaemia and Anaemia Among Pregnant women in Warri, Nigeria
Publication Type journal
Publisher
Publication Authors ISIBOR, C.N.; OMOKARO, E.U.; AHONKHAI I. AND J. A. ISIBOR (2003).
Year Published 2003-10-20
Abstract A total of 319 pregnant women attending the antenatal clinic of Central Hospital, Warri had their haemoglobin levels estimated using standard haematological methods. Forty-seven (14.7%), expectant mothers were found to be anaemic (haemoglobin level less than 9.0g/dl). The Malaria parasites, the agent of malaria and a major cause anaemia in pregnancy were found in 105 (32.9%) expectant mothers, based on the examination of peripheral blood smears. Multigravidae recorded 33.2% prevalence of malaria parasitaemia that was comparable with that among primigravidae. There was a statistically significant relationship between malaria parasitaemia and haemoglobin levels of pregnant women (p < 0.05). This study emphasizes the need for pregnant women to undergo routine haemoglobin estimation considering the deleterious effects of anaemia on them and their foetuses. An early malaria prophylaxis is recommended if a significant increase in haemoglobin levels in the second trimester is to be achieved.
Publication Title Aetiological Spectrum of Postoperative Wound Infections in Delta State
Publication Type journal
Publisher
Publication Authors ISIBOR C.N., AHONKHAI I. and AKERELE, J. (2000).
Year Published 2000-06-19
Abstract Two hundred and fifty swab specimens from post-operative wounds suspected to be infected were cultured for the presence of aerobic bacteria using standard bacteriological techniques. Aerobic bacteria were present in 210 specimens (84%), while 40 specimens (16%) yielded no growth. The following aerobic bacteria in decreasing order of prevalence were isolated Staphylococcus aureus (37.0%), Klebsiella sp. (20.2%), Pseudomonas aeruginosa (14.11%) Escherichia coli (9.30%), Proteus sp. (7.7%), Staphylococcus epidermidis (7.3%) and Streptococcus pyogenes (4.4%). There appears to be a changing pattern in the bacterial flora of post-operative wounds, from the predominant Pseudomonas aeruginosa to Klebsiella sp.
Publication Title Aerobic bacteria associated with otitis media in Warri, Nigeria
Publication Type journal
Publisher
Publication Authors OPHORI, E. ISIBOR, C., and OMONIGHO, S.E (1998).
Year Published 1998-05-10
Abstract Analysis of the middle ear exudates of 150 children (aged 1-10yrs) with otitis media in Warri, Nigeria indicated a bacterial aetiology of this disease. Ear swabs were collected and cultured on suitable laboratory media. Bacteria species isolated included Pseudomonas spp. (22.2%). Staphylococcus epidermidis (18.1%), Staphylococcus aureus (16.7%), alpha-haemolytic streptococci (15.3%), beta-haemolytic streptococci (13.9%), Klebsiella species (4.2%), Proteus species (2.8%) and E. coli (1.4%). Instances of mixed infection occurred in 25 of the ear aspirates.
Publication Title Identification of Phytochemicals with Inhibitory Potential Against Beta-lactamase Enzymes Via Computer Aided Approach.
Publication Type journal
Publisher
Paper Link https://doi.org/10.1016/j.bioorg.2024.107238
Publication Authors Nwokebu, G.N, Adesina B.A, Isibor C.N, Stephen Ayaosi Aigbepue, S.A, Egbo C., Pureaziba,N., Isaac O, Owolade A, Alabere H, Iwuagwu, M Hussein M, Ibrahim A, Balogun T.
Year Published 2024-02-26
Abstract Introduction
Antibacterial drugs have been widely used for the past century to treat diseases, but their efficacy has been limited by multi-resistant pathogens, particularly those that utilize beta-lactamase enzymes. The inhibition of beta-lactamase enzymes holds great promise for reducing the influence of such pathogens.
Objective
This study aims to evaluate the mechanism of inhibition of phytochemicals with antibacterial activity against two classes of beta-lactamases using computational methods. Methods: To achieve this objective, a total of thirty phytochemicals were docked against SHV-1 beta-lactamase and AmpC beta-lactamase after procurement from Protein Data Bank. The pharmacokinetics (ADMET) and density functional theory (DFT) analysis study were also conducted to unravel the nature of the top six most promising compounds on each protein.
Results
The results showed that a significant percentage of the compounds had binding affinities greater than that of avibactam, the positive control. Quercetin-3-O-rutinoside showed the most promising results against SHV-1 beta-lactamase with an affinity of ?9.4 kcal/mol, while luteolin was found to be the most promising candidate against AmpC beta-lactamase with an affinity of ?8.5 kcal/mol. DFT analysis demonstrated the reactivity of these compounds, and the ADMET study indicated that they were relatively safe.
Conclusion
In conclusion, the study's findings suggest that the selected compounds have significant potential to inhibit beta-lactamase and may be used in combination with antibiotics against organisms that produce beta-lactamase. This study provides a basis for further research in a wet-lab setting to validate the result
Publication Title An assessment of the immune status of some stone quarry workers in Ondo state, Nigeria
Publication Type journal
Publisher
Publication Authors Onemu, S.O.; Obeagu, E.I.; Popoola, A.A ; Osuntuyi, M. A. and Isibor, C.N.
Year Published 2024-01-12
Abstract Stone quarry activities in Nigeria are mostly unregulated such that the workers in these quarries are continuously exposed to the inhalation of silica dust. It has been observed that silica dust particles negatively impact the health of stone quarry workers which usually manifest as respiratory difficulties, asthma-like illnesses and other adventitious events of the lungs. The study was designed to evaluate the probable immunological impact of silica dust inhalation from stone crushing by workers. Blood samples were collected from consenting workers and analyzed for total white blood cells and their subsets. Absolute CD4 cells numbers were also determined. The results indicated that neutrophils and eosinophils numbers increased significantly (P < .05) and CD4 counts declined significantly (P < .001). Alteration in these proportions is a pointer to the injurious impact of silica dust on the immune system of these workers. The findings in this study should spur actions in the education of these workers on the need for the use of proper personal protection equipment and the establishment of a scheme to periodically carry out a health assessment check to identity those at most risk of developing chronic illnesses.
Publication Title Experimental Evaluation of the Effects of Ciprofloxacin and Levofloxacin on Semen Parameters of Male Rat
Publication Type journal
Publisher Elite Journal of Laboratory Medicine
Publication Authors Onemu, S.O, Kolawole, J.T, Isibor, C.N and Ademulegun, F.G
Year Published 2024-08-13
Abstract Diverse range of adverse effects in the administration of fluoroquinolones that includes negative impacts on semen parameters abound in literature. The current study evaluated the effects of ciprofloxacin and levofloxacin on the semen indices of experimental rats. Groups of ten rats each administered with standard doses of ciprofloxacin and levofloxacin twice daily for seven days and a third group with sterile distilled water as control. Examination of semen samples from the cauda epididymis on day 35 after the agents’ administration revealed significant declines in sperm cells motility (P<0.0001); significantly increased abnormal sperm cells morphology (P<0.0001) as well significant declines in sperm cells concentrations were recorded for both ciprofloxacin and levofloxacin. This study illuminates the harmful effects of these fluoroquinolones on male fertility. There is therefore, a need for careful assessment of the potential harm versus benefits in the use of these antimicrobial agents, otherwise preference for substitute agents especially in men with fertility challenges is desirable.
Publication Title Isolation and sensitivity patterns of Pseudomonas aeruginosa from nonclinical samples.
Publication Type journal
Publisher African Journal of Biomedical Research
Publication Authors Isibor, C. N. Jemikalajah, D. J , Emegha, J. A. and Ezie, E
Year Published 2024-05-31
Abstract The presence of pathogenic microorganisms in hospital environment possesses obvious clinical risk to patients with some obvious consequences. The current study was conducted to ascertain the prevalence of Pseudomonas aeruginosa in nonclinical samples in central Hospital, Agbor. A total of 25 Pseudomonas aeruginosa were isolated from a total of 110 nonclinical samples collected intermittently from Sinks traps, Mop heads, bed pans from four (4). The samples were inoculated on MacConkey and blood agar plates and incubated at 37 °C for 24 h. The isolates were identified by conventional microbiological tests. Antimicrobial susceptibility pattern was determined by modified Kirby-Bauer disk diffusion method. The overall prevalence of Pseudomonas aeruginosa was 23.8% with a range of 16.2% to 33.2% in the wards examined. The most contaminated area with Pseudomonas aeruginosa was sink traps (36.) %), floors (24.0%), mopheads (20.0%) while bedpans accounted for 20.0% of the isolate sources. the antibacterial susceptibility patterns of the Pseudomonas aeruginosa to ten (10) antibacterial agents used showed 84% susceptibility to pefloxacin, 76% to ofloxacin, while ceftriaxone and ciprofloxacin recorded 72% susceptibility respectively. 64% of the isolates were susceptible to gentamicin. The continued contamination of hospital wards with Pseudomonas aeruginosa highlights the necessity for robust infection control measures, ongoing monitoring and management of the hospital environment to minimize the risk of healthcare-associated infections.
Publication Title Determination of Minimum Inhibitory Concentration of Clinical Isolates from a Hospital in South -South Nigeria.
Publication Type journal
Publisher Journal of Computing , Science & Technology
Publication Authors Isibor, C.N and Jemikalajah Johnson D
Year Published 2024-05-15
Abstract Infections caused by microorganisms have become a significant public health concern in Nigeria, leading to increased morbidity and mortality rates. This study was designed to evaluate the in-vitro activities of three commonly prescribed quinolone and cephalosporin agents against clinical bacterial isolates from Central Hospital Warri. The minimum inhibitory concentrations (MIC) were investigated using the agar dilution method. The invitro activity of quinolones- pefloxacin, ciprofloxacin, ofloxacin were compared with 3 other agents namely ceftazidime, cefuroxime, and gentamicin. Pefloxacin showed greater activity followed by ofloxacin and ciprofloxacin. Overall, the MIC90 values for the quinolones, ciprofloxacin, pefloxacin, and ofloxacin were below 2 mg/l. Pefloxacin and ofloxacin inhibited 90% of isolates of Escherichia coli, Klebsiella sp, Staphylococcus aureus and Proteus sp at values <0.6mg/l. The MIC90 value for ciprofloxacin was higher with 1.76 mg/l as MIC90, for Klebsiella sp in this study. Against Pseudomonas aeruginosa, ofloxacin had MIC90 values of 2.75 mg/l. The MIC90 for Staphylococcus aureus was 0.5, 0.7 and 1.2 mg/l respectively for pefloxacin, ofloxacin and ciprofloxacin. Pefloxacin was therefore adjudged the most active of the quinolone compounds in this study. However, the in-vitro potency of pefloxacin was equivalent to that of ofloxacin which could be prescribed as an alternative, but greater than those of cefuroxime, ceftazidime, and gentamicin. It is therefore pertinent to state that medical laboratories should periodically review their antibiotic usage to curb the resistant issues among organisms.
Publication Title Modeling the transport and fate of waterborne pathogens for enhanced water quality and public health protection
Publication Type journal
Publisher Journal of Biodiversity and Environmental Sciences
Publication Authors Ukpene, A.O, Molua, C.O, Isibor,C.N, Apaokueze, T. N, Vwavware, J.O, Emagbetere, CP. Ukpene "
Year Published 2023-04-27
Abstract This research delves into the intricate dynamics of waterborne pathogens and their influence on water quality
and public health protection. The study's primary objective is to unveil the mechanisms governing the
transport and fate of these pathogens in various water bodies, utilizing a robust methodology that combines
data collection, statistical analysis, mathematical modelling, and geographic information systems. The data
encompass pathogen concentrations, water quality parameters, and other relevant variables collected across
diverse locations, depths, and downstream areas. A rigorous preprocessing and validation process ensures the
quality and integrity of the data, while normalization provides consistency for meaningful analysis. The results
of our study offer illuminating insights into the interactions between water quality parameters and pathogen
concentrations. Statistical analyses reveal significant associations, which have implications for understanding
pathogen behaviour's temporal and spatial trends. Mathematical models, validated against the data, provide a
comprehensive framework for simulating the transport and fate of waterborne pathogens. Spatial analysis
using Geographic Information Systems (GIS) helps pinpoint areas of concern and potential contamination
sources, further enhancing the study's utility. The findings yield practical recommendations for improving
water quality and public health protection, encompassing strategies for mitigating pathogen contamination
and enhancing water quality management. This research advances our knowledge of waterborne pathogen
dynamics and serves as a practical resource for water quality professionals, public health agencies, and
environmental scientists. By elucidating the intricate interplay between pathogens, ecological parameters, and
public health, this study contributes to enhancing water quality and safeguarding public health, reinforcing
the importance of rigorous scientific research in these critical domains.
Publication Title Prevalence of Gram-negative bacteria in Fresh Vegetables on retail in the Open Market in Owo Township, Ondo State, Nigeria
Publication Type journal
Publisher Lifeline Microbiology
Paper Link https://lifelinejournals.org/journals/lifeline-microbiology/volume-2-issue-1-2024-1727861815057
Publication Authors Onemu, S.O; Odeyemi, O,; Egbokhare, J.O and Isibor , C.N
Year Published 2024-10-10
Abstract Fresh vegetables serve as major sources of micronutrients yielding critical vitamins, phytochemicals and essential minerals that improve wellness and supportive in staving-off non-communicable diseases, NCDs. Vegetables are generally cultivated in soils that also contain resident microorganisms that may contaminate them directly or shed from human and animal activities. The current study intended to evaluate fresh raw vegetables on retail in the open market in Owo Township, Ondo State, Nigeria for the presence of the Gram-negative bacterial flora. Twelve samples of six different types of vegetables collected and examined, applying bacteriological techniques for microbial count and assortments of Gram-negative bacteria. The mean bacterial load ranged from 3.66-111.0 x 106 CFU/g and seven genera of Gram-negative bacteria recovered. Klebsiella spp, 22 representing 25% of the isolates was the most dominant microorganism. Escherichia coli 20(22.7%) was the next in frequency from which an isolate serotyped 0157:H7 strain. Other isolates were Enterobacter spp 17(19.3%) Proteus spp 15(17.0%), Pseudomonas aeruginosa 10(11.4%) and two, 2(2.3%) isolates each of Serratia marcescens and Salmonella enterica. The isolates were most susceptible to ciprofloxacin and levofloxacin, and least to amoxicillin-clavulanate. All the isolates were resistant to amoxicillin and tetracycline. Extended-spectrum beta lactamases were detectable in five of the seven genera (2.3-4.5%) excluding Serratia marcescens and Samlonella enterica isolates. This study exposed evidence of vegetables pollution with microorganisms associated with foodborne outbreaks of illnesses, compelling an urgency for education in handling practices compatible with the safety of fresh vegetables and related farm produce.
Publication Title Bacteriological Assessment of Restaurants Environment in Abraka, South-South, Nigeria
Publication Type journal
Publisher FUPRE Journal of Scientific and Industrial Research
Publication Authors Isibor, C. N. , Jemikalajah, D. J. , Ikunbor, F. , Iliaya, J. Z , Isibor, J. C , Nwafulugo, C. E.
Year Published 2024-10-26
Abstract This study investigated the prevalence and distribution of bacterial contamination
in five different restaurants in Abraka, Delta State, Nigeria. Samples were
collected randomly from various surfaces, including the hands and aprons of food
handlers,Floor, and doorknobs. Standard microbiological methods was used for
the examination of the specimens in the laboratory to identify the bacterial species.
The results showed that Staphylococcus aureus was the most common bacterial
isolate, comprising 36.14% of all isolates. Escherichia coli followed closely behind
at 20.18%, while Streptococcus spp accounted for 10.84%. Pseudomonas aeruginosa
and Bacillus sp were present at 10.24% and 7.23%, respectively. Salmonella sp and
Klebsiella spp were found at 4.52% each, while Proetus sp and Enterobacter sp
made up 3.31% and 3.01% of the isolates, respectively. The restaurant
environment with the highest percentage of isolated contamination was Restaurant
C, with a recorded value of 27.11%. Following closely behind were Restaurant B
with 23.80%, Restaurant D with 18.07%, and Restaurant A with 16.27%. On the
other hand, the restaurant with the least contamination was Restaurant E, with a
recorded value of 14.76%. This study therefore indicates some level of bacterial
contamination in the restaurants which could have significant implications for
public health and food safety. We therefore recommends regular monitoring of
bacterial contamination in food outlets in addition to the implementation of
effective hygiene protocols to minimize the risk of foodborne illness in Abraka
environment.
Publication Title Antibacterial Activity of Lemon Juice Extract Against Pseudomonas aeruginosa and Escherichia coli
Publication Type journal
Publisher International Research Journal of Pharmacy and Medical Sciences
Publication Authors PrJemikalajah, D.J., Isibor, C. N. and Onoharigho, Q
Year Published 2024-11-16
Abstract This study investigated the antibacterial activity of aqueous and ethanol dilutions of lemon juice against Pseudomonas aeruginosa and Escherichia coli, utilizing standard microbiological techniques. The antibacterial efficacy was assessed by measuring the zones of inhibition produced by different concentrations of the dilutions. Results demonstrated a concentration-dependent increase in antibacterial activity. For the ethanolic dilution, Escherichia coli exhibited zones of inhibition of 15.5 mm, 5 mm, 4.5 mm, 4 mm, and 4.5 mm, while Pseudomonas aeruginosa showed zones of 16 mm, 15 mm, 13 mm, 9.5 mm, and 7.5 mm at concentrations of 100mg/ml, 75mg/ml, 50mg/ml, 25mg/ml, and 12.5mg/ml, respectively. The aqueous dilution also showed antibacterial activity, with Escherichia coli with zones of 13 mm, 10 mm, 6.5 mm, 2 mm, and 1.5 mm, and Pseudomonas aeruginosa zones of 9 mm, 6 mm, 9 mm, 8 mm, and 4 mm at the same concentrations. The minimum inhibitory concentration (MIC) of the ethanolic dilution was found to be 50mg/ml for Escherichia coli and 25mg/ml for Pseudomonas aeruginosa, while the aqueous dilution observed was 100mg/ml concentration to inhibit both bacteria. These findings suggest that lemon juice dilution, particularly ethanolic, have potential as natural antibacterial agents