Publication Title Hematological and biochemical parameters in chinchilla rabbits treated with Caulis bambusae (Bamboo) stem extract
Publication Type journal
Publisher Scholars Research Library
Publication Authors Oshilonyah, H.U., Ibeh I.N., Nwachukwu, N.C. and Ijioma, S.N.
Year Published 2015-05-05
Abstract This study investigated the effects of Caulis bambusae stem extract on haematological and biochemical parameters rabbits to ascertain possible toxicity. Twelve aged matched healthy adult male chinchilla rabbits (1.80 ± 0.05–2.0 ± 0.11kg body weight) were divided into three groups. Group 1 was given distilled water in addition to normal feed and served as the control. Groups 2 and 3 were treated with 2.5 and 5ml of aqueoue extract of Caulis bambusae by oral intubation. Treatment lasted 90 days with blood collected on days 30, 60.And 90 for analysis. Results obtained revealed no significant difference (P>0.05) in red blood cells (RBC), haemoglobin (Hb), Packed cell volume (PCV), Mean corpuscular volume (MCV), Mean corpuscular haemoglobin (MCH) and Platelets counts between the treatment groups and control. White blood cell count was significantly (P<0>0.05) when compared to the control. Total cholesterol (TC), Triglycerides (TG), High density lipoprotein cholesterol (HDL-C) and Low density lipoprotein cholesterol (LDL-C) were however significantly decreased (P<0.05) in the treatment groups. The results suggest thatCaulis bambusae stem extract has no adverse effect on the haematological and biochemical profile of treated experimental animals but showed evidence of hypolipidaemic activity which could be of value in the management of cardiovascular problems.
Publication Title Phytochemical analysis and preliminary in Vitro non mutagenic activity of Caulis bambusae stem extract.
Publication Type journal
Publisher International Journal of Healthcare Sciences ISSN 2348-5728 (Online)
Paper Link www.researchpublish.com
Publication Authors 1,2 Oshilonya, H.U., 3Oshilonya, L.U., 4Ijioma, S.N
Year Published 2017-07-07
Abstract In this study, the phytochemical composition and mutagenic potential of Caulis bambusae stem extract were investigated following standard procedures, with a view to ascertain its health implications and possible deleterious effects on body cells. Results of the phytochemistry showed that Caulis bambusae stem extract contains flavonoids (0.09%), alkaloids (0.05%), tannins (2.10mg/dl), steroids (1.40mg/dl), carbohydrates (22.30%), anthraquinone (0.03%), glycosides (0.14%), and saponins (1.75mg/dl), while the mutagenicity study on characterized E. coli strain showed no significant increase (P>0.05) in induced tryptophan revertant colonies and a mutagenic index of 1.00±0.12?g/plate when compared to the positive control, indicating absence of mutagenic potential. The significant presence of these phytochemical agents in Caulis bambusae suggests that the plant is highly enriched with bioactive substances which can be harnessed into safe and potent medicines for the treatment of diseases and also gives credence to the use of the plant in ethno medicine for the treatment of various ailments.
Publication Title Prevalence of type-2 diabetes mellitus amongst suspected subjects in Agbor, Delta State, Nigeria
Publication Type journal
Publisher Scholars Research Library
Publication Authors Oshilonyah, H.U., Ijioma, S.N. and Ibeh, I.N.
Year Published 2015-05-05
Abstract • The prevalence of type 2 diabetes mellitus amongst suspected persons in Agbor, Delta State, Nigria, and its relationship with age and gender was studied. Two hundred suspected subjects comprising of 100 males and 100 females of ages 30 and above who were referred to Government Hospital, Owa-alero, Agbor, were screened using a Glucose meter (Randokit) following standard procedures prescribed by the producer. Individuals whose fasting blood sugar levels were above 180mg/dl after three visits were considered to be diabetic. Results indicate that 118 subjects out the suspected 200 were found to be diabetic representing a total prevalence rate of 59%. The risk of developing diabetes mellitus was also found to be increasing with increasing age with females being more susceptible to developing the disease than females. Those of age 30-50 years old had incidence rates of 9.1% and 56.86% for males and females respectively, as those 51-70 years old had incidence rates of 48.39% and 82% for males and females respectively while those 71 years and above had incidence rates of 59.26% and 100% for males and females respectively. The development of diabetes mellitus in Agbor, Delta State, Nigeria is therefore strongly associated with increasing age and females at all age brackets above 30 are at higher risk of developing the disease than their male counterparts.
Publication Title Hypoglycaemic and hypolipidemic effects of alcoholic extract of common sage (Salvia Officinalis) in streptozotocin-induced diabetic rabbits.
Publication Type journal
Publisher African Journal of Biomedical Research
Publication Authors Mokogwu A.T.H, Adjekuko C.O.,Oshilonyah, H.U., Ikpefan J.O., Eyenubo O.B., Avwioro O.G.
Year Published 2022-02-02
Abstract Objective: To evaluate the hypoglycaemic and hypolipidemic effect of alcoholic extract of common sage (Salvia officinalis) on streptozotocin-induced diabetic rabbits. Methods: Twenty five male rabbits of weight 950g- 1150g were used for the study. The animals were grouped into 5 of 5 rabbits each. Group A- Diabetic control, Group B - Diabetic rabbits treated with glibenclamide (0.25 mg/kg), Group C - Diabetic rabbits treated with alcoholic leaf extract at 250 mg/kg and Group D - Diabetic rabbits treated with the leaf extract at 500 mg/kg. Group E- served as normal non-diabetic control. The animals were given a standard diet and water ad libitum. Diabetes was induced in the rabbits by a single intra-peritoneal injection of freshly prepared streptozotocin 50 mg/kg b.w. followed by 120 mg/kg of nicotinamide in 0.1 M citrate buffer, pH 4.5 in a volume of 0.5 ml/kg b.w. Blood samples were collected on the 1st, 7th, 14th and 21st days from the marginal ear vein of the animals and analysed for blood glucose and lipid profile levels. Results: The standard/investigational drugs treated rabbits showed a marked decrease in the level of blood glucose when compared with the diabetic control at a significant level of p < 0.001. The alcoholic extract of common sage leaf (at 250mg/dl) showed a significant decrease (p < 0.05) compared with the diabetic control rabbits. Equally, the leaf extract (at 500mg/dl) showed a significant decrease (p < 0.001) in the blood glucose levels when compared with the diabetic control group. The administration of the leaf extract at 250mg/dl and 500mg/dl to the animals prevented gross alterations in the lipid profile levels. Conclusion: The alcoholic leaf extract of common sage (Salvia officinalis) has hypoglycaemic and hypolipidemic activity on streptozotocin -induced diabetic rabbits.
Publication Title Study of Salvia Officinalis leaf extract on some biochemical parameters in rats induced with overdosed-tramadol.
Publication Type journal
Publisher African Journal of Biotechnology
Publication Authors Mokogwu A.T.H, Adjekuko C.O.,Oshilonyah, H.U., and Avwioro O.G
Year Published 2022-02-02
Abstract The aim of the study was to evaluate the effect of Salvia officinalis leaf extract in overdosed-tramadol induced rats. Standard methods of analysis were used for the study. Fifty rats were divided into Group A (Control) - feed and water only, Group B-induced with tramadol at 50 mg/kg body weight, Group Cinduced with tramadol + 150 mg/kg of S. officinalis leaf extract, Group D-tramadol + 300 mg/kg S. officinalis leaf extract, and Group E-tramadol + 400 mg/kg S. officinalis leaf extract. The results showed high concentrations of phytochemical and trace elements at various concentrations. There were increases in aspartate amino transaminase, alanine aminotransferase, gamma gluamyltransferase, alkaline phosphatase and in total protein, albumin, total bilirubin, conjugated bilirubin, sodium, potassium, chloride, bicarbonate, urea, calcium, creatinine, total cholesterol, magnesium, hemoglobin, packed cell volume, and total white blood cell count at a significant difference (p ? 0.05) in Group B when compared with Group A. However, in Groups C, D and E, there was statistically significant decrease (p ? 0.05) in aspartate amino transaminase, alanine aminotransferase, gamma gluamyltransferase, alkaline phosphatase and total protein, albumin, total bilirubin, conjugated bilirubin, sodium, potassium, chloride, bicarbonate, urea, calcium, creatinine, total cholesterol and magnesium compared with the Group B. The study showed that S. officinalis leaf could be of an unalloyed health benefits in the management of tramadol-induced toxicity in rats.